rnase free dh2o (TaKaRa)

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TaKaRa rnase free dh2o
Rnase Free Dh2o, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 3731 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rnase+free+dh2o/RNase+A/pm41252873-60-21-31
Average 97 stars, based on 3731 article reviews

rnase free dh2o - by Bioz Stars, 2026-09

97/100 stars

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Article Title: Vascularized Bioengineered Kidney Using Decellularized Scaffold Recellularized with human Placenta-Derived Angiogenic stem Cells and Kidney Organoids
Article Snippet: The remaining supernatant was dried for 5 minutes and resuspended in 30 μl RNase-free dH2O (Takara, RR047A).

Article Title: Temporin-1CEa and its Analog LK2(6)A(L) Confer Neuroprotective Effects in Parkinson's Disease Model by Attenuating Neuroinflammation Via the NF-κB and MAPK Signaling Pathways.
Article Snippet: The primers, SYBR Premix Ex TaqII, cDNA, and RNase Free dH2O in a 20 μL reaction mixture were prepared according to the manufacturer’s instructions (RR420A, Takara, Dalian, China).

Article Title: Sea Cucumber Derived Triterpenoid Glycoside Frondoside A: A Potential Anti-Bladder Cancer Drug.
Article Snippet: PrimeScriptTMRT reagent Kit with gDNA Eraser (Perfect Real Time), TB Green® Premix Ex TaqTM II (Tli RNaseH Plus), and RNase Free dH2O were obtained from TaKaRa Bio (TaKaRa, Dalian, China).

Reverse Transcription:

Article Title: Evaluation of Lysophosphatidic Acid Effects and Its Receptors During Bovine Embryo Development.
Article Snippet: After RNA isolation, reverse transcription was conducted using the PrimeScriptTM RT reagent Kit (Takara, Tokyo, Japan). .. The reverse transcription (RT) mixture contained 10 μL of the RNA sample, 1 μL of PrimeScript RT Enzyme Mix I (Takara), 1 μL of RT Primer Mix (Takara), 4 μL of 5×PrimeScript Buffer 2 (Takara) and 4 μL of RNase-free dH2O (Takara), in a total volume of 20 μL. .. Gene-specific primer pairs (Sangon Biotech, Wuhan, China) were designed using Primer Premier (v5.0) and the NCBI primer-blast tool (Table 2).

Polymerase Chain Reaction:

Article Title: Hesperetin improves diabetic coronary arterial vasomotor responsiveness by upregulating myocyte voltage-gated K + channels.
Article Snippet: .. Total RNA was converted into cDNA in a 20 μl PCR reaction system with 10 μl reaction system from the previous step, 1 μl PrimeScript RT Enzyme Mix I, 1 μl RT Primer Mix, 4 μl 5X Primer Script Buffer 2, 4 μl RNase Free dH2O (PrimeScriptTM RT reagent kit with gDNA Eraser; Takara Bio, Inc.). .. C1000 TouchTM Thermal cyclers (Bio-Rad Laboratories, Inc.) were used to perform RT with the following heat cycle: 37 ̊C for 15 min and 85 ̊C for 5 sec. A total of 2 μl cDNA was amplified in a 23 μl reaction system containing 12.5 μl SYBR® Premix Ex TaqTM (Takara Bio, Inc.), 8.5 μl DEPC‐treated water, 1 μl forward primer (10 μM) and 1 μl reverse primer (10 μM).

Synthesized:

Article Title: S100A8 and S100A9-mediated keratinocyte affecting T lymphocyte immune imbalance through TLR4/NF-κ B in psoriasis.
Article Snippet: Psoriasis is a chronic,immune-mediated inflammatory skin disorder characterized by recurrent thick plaque.. As an alarmin of inflammation, the importance of S100A8 and S100A9 have already been confirmed to be associated with the development of chronic inflammation in diseases.. However, the precise mechanisms of S100A8 and S100A9 in psoriasis remain unclear.

Article Title: Histone methyltransferase KMT2D mediated lipid metabolism via peroxisome proliferator-activated receptor gamma in prostate cancer.
Article Snippet: .. Then, cDNA was synthesized from 500 ng RNA of each sample, 2 μL PrimeScript RT Master Mix (Takara Bio, Otsu, Japan), and RNase-free dH2O (Takara Bio, Otsu, Japan), which was added to a final volume of 10 μL. .. The total volume (10 μL) of each PCR reaction mixture contained 2.5 μL SYBR Green Supermix (Takara Bio, Otsu, Japan), 1 μL RNase-free dH2O, 1 μL cDNA, and 10 μM of each of forward and reverse primers.

Article Title: High-fat diets provoke phosphorus absorption from the small intestine in rats.
Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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